P106H - Herpes Simplex Virus (HSV) II Nucleic Acid Detection Kit
Tianlong
China
1-2 weeks
50000 Test/M
The kit is used for qualitative detection of HSV II nucleic acid in male urethral swab samples or female cervical swab samples.
Herpes simplex belongs to the subfamily of the herpesvirus family, and the size of the virus plasmid is about 180nm. The HSV genome is approximately 152kb, with 34 genes, encoding more than 70 polypeptides. HSV II mainly causes genital infections below the waist, which are transmitted through sexual contact.
Product Name:
Herpes Simplex Virus (HSV) II Nucleic Acid Detection Kit (Fluorescence PCR Method)
Spec:
32T/kit
Intended Use:
The kit is used for qualitative detection of HSV II nucleic acid in male urethral swab samples or female cervical swab samples.
Herpes simplex belongs to the subfamily of the herpesvirus family, and the size of the virus plasmid is about 180nm. The HSV genome is approximately 152kb, with 34 genes, encoding more than 70 polypeptides. HSV II mainly causes genital infections below the waist, which are transmitted through sexual contact.
The laboratory test methods of HSV II include:
1) Cytology;
2) Serological testing;
3) Antibody and antigen detection;
4) Nucleic acid amplification test.
The kit provided is suitable for the auxiliary diagnosis of HSV II. The test results of this kit are for clinical reference only and cannot be used as the basis for diagnosis or exclusion of cases.
Principle of Test:
The highly conserved sequences of HSV II are selected to design specific primers and Taqman probes. The probes can specifically bind to a DNA template in the middle of the primer amplification region. In the process of PCR extension, the exonuclease activity of Taq enzyme will cut off the 5’end fluorescent groups from the probes, freeing them in the reaction system, thus breaking away from the shielding of the 3’end fluorescence quenching groups, which can receive light stimulation and emit fluorescence that can be detected by the instrument, so as to achieve the automatic detection of HSV II nucleic acid in closed reaction system.
A synthetic non-competitive sequence is designed as an internal control template in this kit, which has no interference with the target gene of HSV II, then BLAST ratio analysis is performed for this sequence on NCBI website, confirmed that it cannot be found in the NCBI nucleic acid library, no interfere with each other. The primers and probes are designed according to the internal control template, the internal control adopts VIC channel, so as to realize the detection process monitoring in closed reaction system, which can effectively monitor the occurrence of false negative results.
Storage Condition & Validity:
All reagents should be stored at -20ºC±5ºC. The validity period of the reagents is 12 months. The unused reagents can be sequentially cryopreservation without affecting their stability. The regents could be preserved for 30 days at -20ºC±5ºC after repeated thawing and freezing three times. The opened reagents could be placed at room temperature for no more than 8 hours. The products should be transport by ice box or refrigerated truck. Simulated transport tests indicate that the stability and validity could not be influenced by transport, but the transportation time should not exceed 7 days. Production date and expiration date are marked on the label.
Applicable Instrument:
Gentier 96E real time PCR system, Gentier 96R real time PCR system and Gentier 48E real time PCR system,ABI7500 real time PCR systems or similar apparatus .
Sample Requirements:
1. Sample: male urethral swab samples or female cervical swab samples.
2. Collection container: disposable male swabs or female swabs approved by the state should be used. The swabs should include sleeves, cotton swab rods, and stoppers. The cotton swab rods and stoppers should be firmly connected.
3. Collection: for specific sampling method, please refer to the ‘Microbial Specimen Collection Manual’.
3.1 Male: clean the urethral orifice first, and the patient is required not to urinate for 2 hours before sampling. Take urethral secretions or a small cotton swab to extend into the urethra 2~4cm, slightly twist the swab to remove the secretion (it should be slightly mucous), place the secretion or cotton swab into a sterile glass tube and seal it for inspection.
3.2 Female: first use a cotton/linen swab to wipe off the excessive secretions of the cervix, use a vaginal dilator to expand the vagina, then exchange for a cotton swab to extend into the cervix and pass through the epithelial junction until the swab head cannot be seen, rotate for 10-20 seconds, take out the swab, put the swab in a sterile glass tube and seal it for inspection.
4. Storage: samples can be stored at 2~8ºC for no more than 24 hours; under -20ºC for no more than 3 months; under -70ºC for long-time, but repeated freeze-thaw should be avoided.
5. Transportation: use a curling pot with ice or a foam box with ice to seal for transportation.
FAQ
What is the difference between Gentier96 and Gentier48 ?
1.1 Sample throughout difference: Gentier96 can process maximum 96 samples per run, Gentier48 can process maximum 48 samples per run; 1.2 Fluorescence channel difference: Gentier96 E/R has 6/4 fluorescence channels separately, Gentier48 E/R has 4/2 fluorescence channels; 1.3 Light source location difference : The light source of Gentier96 is on the top,