Vaccination rates and the incidence of cervical adenocarcinoma are both on the rise, making HPV genotype testing an important tool to help stratify risk and guide patient management. High-risk HPV genotypes 16, 18 account for 70% of cervical cancer worldwide. Tianlong’s Human Papillomavirus (HPV) Nucleic Acid Detection Kit is a qualitative in vitro test for detection of 18 high-risk HPV types(HPV 16,18,26,31,33,35,39,45,51,52,53,56,58,59,66,68,73 and 82), which can specifically identify types HPV 16 and HPV 18 while concurrently detecting the rest of other high-risk types.
Coefficient of variation (CV%) of Ct value ≤5%
Collocating with Tianlong extraction reagent makes your experiment results more accurate
Applicable in instruments with FAM 、HEX/VIC、CY5 and TEXAS RED/ROX channels
Drive laboratory efficiency
Detection of 18 high-risk HPV types in one run, specifically identifies types HPV 16 and HPV 18
Individual genotype reporting of 2 HR-HPV types
Concurrently detects remaining 16 HPV genotypes
26,31,33,35,39,45,51,52,53,56,58,59,66,68,73 ,82 Positive or Negative Pooled Result
Principle of Test:
The highly conserved sequences of ureaplasma urealyticum are selected to design specific primers and Taqman probes. The probes can specifically bind to a DNA template in the middle of the primer amplification region. In the process of PCR extension, the exonuclease activity of Taq enzyme will cut off the 5’end fluorescent groups from the probes, freeing them in the reaction system, thus breaking away from the shielding of the 3’end fluorescence quenching groups, which can receive light stimulation and emit fluorescence that can be detected by the instrument, so as to achieve the automatic detection of ureaplasma urealyticum nucleic acid in closed reaction system. A synthetic non-competitive sequence is designed as an internal control template of this kit, which has no interference with the target gene of ureaplasma urealyticum, then BLAST ratio analysis is performed for this sequence on NCBI
website, confirmed that it cannot be found in the NCBI nucleic acid library, no interfere with each other. The primers and probes are designed according to the internal control template, the internal control adopts HEX channel, so as to realize the detection process monitoring in closed reaction system, which can effectively monitor the occurrence of false negatives.
Storage Condition & Validity:
All reagents should be stored at -25ºC~-15ºC. The validity period of the reagents is 12 months.
The unused reagents can be sequentially cryopreservation without affecting their stability, but it should not exceed three times for repeated thawing and freezing. The opened reagents should be placed at room temperature under the condition of no more than 8 hours. The products should be transport by ice box or refrigerated truck. Simulated transport tests indicate that the stability and validity could not be influenced by transport, but the transportation time should not exceed 7 days. Production date and expiration date are shown on the packaging label.
ABI7500 real time PCR systems, TL988-IV real time PCR system, Gentier 96E real time PCR system, Gentier 96R real time PCR system and Gentier 48E real time PCR system.
Sample Requirements :
1. Sample: male urethral swab samples or female cervical swab samples.
2. Collection container: disposable male swabs or female swabs approved by the state should be used. The swabs should include sleeves, cotton swab rods, and stoppers. The cotton swab rods and stoppers should be firmly connected.
3. Collection: for specific sampling method, please refer to the ‘Microbial Specimen Collection Manual’.
3.1 Male: clean the urethral orifice first, and the patient is required not to urinate for 2 hours before sampling. Take urethral secretions or a small cotton swab to extend into the urethra 2~4cm, slightly twist the swab to remove the secretion (it should be slightly mucous), place the secretion or cotton swab into a sterile glass tube and seal it for inspection.
3.2 Female: first use a cotton/linen swab to wipe off the excessive secretions of the cervix, use a vaginal dilator to expand the vagina, then exchange for a cotton swab to extend into the cervix and pass through the epithelial junction until the swab head cannot be seen, rotate for 10-20 seconds, take out the swab, put the swab in a sterile glass tube and seal it for inspection.
4. Storage: samples can be stored at 2~8ºC for no more than 24 hours; under -20ºC for no more than 3 months; under -70ºC for long-time, but repeated freeze-thaw should be avoided.
5. Transportation: use foam box and ice bag to seal for transportation.
What is the difference between Gentier96 and Gentier48 ?
1.1 Sample throughout difference: Gentier96 can process maximum 96 samples per run, Gentier48 can process maximum 48 samples per run; 1.2 Fluorescence channel difference: Gentier96 E/R has 6/4 fluorescence channels separately, Gentier48 E/R has 4/2 fluorescence channels; 1.3 Light source location difference : The light source of Gentier96 is on the top,